WIL2 S
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Cat. No.: CL-1039
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CL-1039
$ 600.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | WIL2 S |
| Synonyms | WIL2-S;WIL2/S;WIL2S;WIL2 Secreting |
| Cellosaurus Accession | CVCL_3809 |
| Organism | Human |
| Growth Properties | Aggregated suspension with loose adherence |
| Morphology | Lymphoblast-like |
| Tissue | Spleen |
| Age | 5Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Both single cells and cell clusters are present in suspension. A small proportion of cells may adhere and can be dislodged by gentle pipetting. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | 1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | WIL2-S is a human B lymphocyte cell line isolated from the spleen of a 5-year-old White male patient with hereditary spherocytosis. As a HAT-sensitive variant of the parental WIL2 human B-cell line, it serves as a suitable host for transfection experiments. |
| Biosafety Level | BSL-2 |
MSDS
