WM115

Name: WM115
Brand: Procell
SKU: CL-0914
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0914

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CL-0914

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	WM115
Synonyms	WM-115;WM 115;WM115F;WM115-mel;WM115mel;WC00079
Cellosaurus Accession	CVCL_0040
Organism	Human
Growth Properties	Adherent
Morphology	Fibroblast-like
Disease	melanoma cells
Tissue	Skin; derived from metastatic site: thigh
Age	55Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	DMEM [PM150210]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	5-7 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

WM115 cells are a tumorigenic (VGP) primary melanoma cell line with metastatic potential. This cell line was established from a metastatic site (right anterior leg skin) in a 55-year-old female with superficial spreading melanoma. The subject had a VGP tumor with Clark level III and a thickness of 2.24 mm. This cell line harbors a specific V600D (Val600Asp) mutation at codon 600 of the BRAF gene. It also exhibits PTEN loss of function, including hemizygous deletion. This cell line is derived from the same patient as the cell lines WM239A, WM165-1, and WM266-4. WM115 originated from a skin metastasis, while WM239A, WM165-1, and WM266-4 were derived from individual lymph-node metastases. WM115 is wild-type for the N-RAS, c-KIT, and CDK4 genes. When injected into immunocompromised mice, WM115 cells form xenograft tumors.

Biosafety Level

BSL-1