Z-138
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Cat. No.: CL-0955
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CL-0955
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | Z-138 |
| Synonyms | Z138 |
| Cellosaurus Accession | CVCL_B077 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Tissue | Bone marrow |
| Age | 70Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | IMDM [PM150510]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:5 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | Z-138 is a lymphoblastoid cell line established in 1990 from a Caucasian male patient initially diagnosed with chronic lymphocytic leukemia. Approximately two years later, the neoplasm transformed to aggressive mantle cell lymphoma. This cell line is suitable for use in immunological research. |
| Antigen Expression | CD3-、CD5-、CD10-、CD19+、CD20+、CD23+、FMC7- |
| Gene Expression | CD3-；CD5-；CD10-；CD19+；CD20+；CD23+；FMC7- |
| Biosafety Level | BSL-1 |
MSDS
