Cell Culture Fundamentals - A Comprehensive Guide

This guide presents key strategies to safeguard experiments: mastering aseptic technique, maintaining personal and environmental hygiene, caring for equipment such as biosafety cabinets and CO₂ incubators, ensuring reagent sterility, and authenticating cell lines. It also offers practical workflows for observation, rapid screening, diagnostic testing, and corrective actions. Together, these measures provide a structured framework to minimise contamination risk and protect experimental integrity.