When and How to Subculture Cells | Lab Techniques Simplified

Nov 25,2025

This video covers the essential techniques for subculturing cells, a critical skill for maintaining healthy cultures. Learn when and why subculturing is necessary, how to identify the right time to subculture, and step-by-step methods such as direct medium addition and centrifugation. Additionally, we provide tips for working with adherent cells, including protocols for detaching and splitting them, ensuring optimal growth conditions.

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