PB180325
$ 40.00
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Product Introduction
Background
HEPES is all known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, is a commonly used biological buffer. pKa (25℃) is 7.5 and an effective buffer range of pH is 6.8-8.2. The greatest advantage of HEPES is that to maintain the pH range of a solution for a longer period of time in an open environment.
HEPES cannot pass through biofilms. It is a chemical and enzymatic stable substance with limited effect on biochemical reactions, and the absorption of visible and ultraviolet light is extremely low. Therefore, it is generally considered that HEPES is an excellent biological buffer, and there is no toxic effect to the cells. The working concentration is 10-50 mM and the final concentration for cell culture is 10 mM.
Ingredient Statement
With
• 238.3 g/L HEPES
• Purified Water Dissolvent
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution.
4. This product is 50 × concentrated solution and dilute it to to the desired concentration before use.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's 1 M HEPES Solution complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1 M |
| Volume | 100mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months |
| Shipping Condition | Ice bag |
Publications
Journal: Advanced Science (2023) IF: 15.100
Product Cited: DMEM/F12, 1 M HEPES Solution, AML12 Cells Complete Medium, RPMI-1640
Journal: JOURNAL OF HAZARDOUS MATERIALS (2023) IF: 13.600
DOI: 10.1016/j.jhazmat.2023.131262
Product Cited: RPMI-1640, 200 mM L-Glutamine Solution, 100×, β-Mercaptoethanol, 1 M HEPES Solution, MEM Non-Essential Amino Acid Solution (NEAA), 100×
Journal: Clinical Respiratory Journal (2023) IF: 1.7
DOI: 10.1111/crj.13665
Product Cited: 1 M HEPES Solution
Journal: MOLECULAR CANCER RESEARCH (2022) IF: 6.333
DOI: 10.1158/1541-7786.MCR-22-0231
Product Cited: 1 M HEPES Solution
Journal: Viruses-Basel (2022) IF: 5.800
DOI: 10.3390/v14112546
Product Cited: 1 M HEPES Solution
