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CL-0014
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Product Introduction
| Cell Name | A-375 |
| Synonyms | A 375;A375;A375-MEL;A375-mel;A375mel |
| Cellosaurus Accession | CVCL_0132 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | melanoma cells |
| Tissue | Skin |
| Age | 54Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | DMEM [PM150210]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:6 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | A-375 cells were derived from a 54-year-old female patient and represent one of a series of cell lines established by D.J. Giard and colleagues. A-375 cells form rapidly growing subcutaneous tumors resembling malignant melanoma in NIH Swiss mice treated with anti-thymocyte globulin and serum; they also form colonies on fibroblast feeder layers as well as in soft agar. |
| Tumorigenic | Yes, in immunosuppressed mice. |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: IEEE TRANSACTIONS ON NANOBIOSCIENCE (2024) IF: 3.9
Product Cited: A-375 Cells Line
Journal: Scientific Reports (2024) IF: 3.8
DOI: 10.1038/s41598-024-80603-y
Product Cited: A-375 Cells Line, DMEM (High glucose) Medium
Journal: LANGMUIR (2024) IF: 3.7
DOI: 10.1021/acs.langmuir.4c01496
Product Cited: A-375 Cells Line
Journal: Frontiers in Oncology (2024) IF: 3.5
DOI: 10.3389/fonc.2024.1471267
Product Cited: Hs 895.T Cells Line, WM793 Cells Line, HS-27A Cells Line, Hs 919.Sk Cells Line, A2058 Cells Line, SK-MEL-2 Cells Line, SK-MEL-28 Cells Line, Hs 895.B Cells Line, RPMI-7951 Cells Line, Hs 936.Sk Cells Line, Hs 895.D Cells Line, UACC-62 Cells Line, C32 Cells Line, Hs 852.T Cells Line, CCD-1140Sk Cells Line, CCD-1121Sk Cells Line, YUGEN8 Cells Line, CCD-1112Sk Cells Line, Hs 895.O Cells Line, Hs 888.Sk Cells Line, IPC-298 Cells Line, A-375 Cells Line, Hs 895.Sk Cells Line, G361 Cells Line, Hs 895.C Cells Line, Hs 895.A cell Cells Line, CCD-1106 KERTr Cells Line, LOX IMVI Cells Line, SK-MEL-5 Cells Line, WM35 Cells Line, MeWo Cells Line, Hs 895.R Cells Line, SK-MEL-3 Cells Line, Hs 895.P Cells Line, Malme-3M Cells Line, WM1552C Cells Line, CCD-1152Sk Cells Line, HMCB Cells Line, CCD-8Sk Cells Line, UACC-257 Cells Line, HS294T Cells Line
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY (2024) IF: 3.5
DOI: 10.1007/s11010-024-05045-6
Product Cited: Penicillin-Streptomycin Solution, 100 ×, SK-MEL-1 Cells Line, A-375 Cells Line, A875 [A-875] Cells Line, HFF-1 Cells Line, SK-MEL-28 Cells Line, Fetal Bovine Serum
