Publications(2)
Manual
PM153312
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Product Introduction
Background
Advanced DMEM/F12 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F12, serum supplementation can be reduced by 50–90% with no change in cellular prolife ratio or morphology. Many cell lines do not need to be domesticated to use this medium. Cells successfully cultured in Advanced DMEM/F12, with no adaptation, include Vero, LX-2, KGN, C8-D1A, Hela, 4T1, NIH-3T3, KLE, ARPE-19, JHH-7, NCI-H2009, etc.
Advanced DMEM/F12 medium can achieve the serum-reduced effect due to the addition of the following ingredients: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin and various trace elements (sodium selenite, ammonium metavanadate, copper sulfate and manganese chloride).
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 365 mg/L L-Glutamine
• 110 mg/L Sodium Pyruvate
• 8.1 mg/L Phenol red
• NEAA
• Purified Water Dissolvent
Without
• HEPES
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. Not all cells are suitable for reduced serum culture. Be sure to test the effect in a small amount before replacing it. If necessary, the FBS concentration should be optimized for each cell line to obtain maximum serum reduction.
3. This product has been filtered and sterilized. Pay attention to aseptic operation to avoid contamination.
4. To maintain the best use of this product, do not perform freeze-thaw treatment.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Advanced DMEM/F12 complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.0-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 12 months |
| Storage Condition | This product can be stored at 2-8°C for 12 months with shading light. |
| Shipping Condition | Ice bag |
Related Products
Publications
Journal: Cell Death & Disease (2025) IF: 9.6
DOI: 10.1038/s41419-025-08093-y
Product Cited: MCF 10A Cell Complete Medium, Advanced DMEM/F12 Medium, Earle's Balanced Salt Solution (EBSS), with phenol red, without calcium, magnesium, JIMT-1 Cells Line, AU565 Cells Line, Leibovitz's L-15 Medium, Fetal Bovine Serum
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY (2025) IF: 4.7
DOI: 10.1016/j.ejphar.2025.178375
Product Cited: Fetal Bovine Serum, Advanced DMEM/F12 Medium
FAQs
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Q:1 My cells are growing very slowly. What could be the cause of this?
AnswerPlease see some common reasons below, and solutions to the issue: - Growth medium is not correct: Use pre-warmed growth medium as recommended by the supplier. - Serum in the growth medium is of poor quality: Use serum from a different lot. and choose good quality serum to ensure nutrition. - Cells have been passaged too many times: Use healthy, low-passage number cells. - Cells were allowed to grow beyond confluency: Passage mammalian cells when they are in the log-phase before they reach confluency. - Culture is contaminated with mycoplasma: Discard cells, media, and reagents. Obtain new stocks of cells, and use them with fresh media and reagents.
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Q:2 What factors can contribute to rapid cell death/culture failure?
AnswerThere are a number of events that can contribute to this: 1. Incorrect CO2 levels:Monitor the level of CO2 manually with a Fyrite kit, available from Bacharach. Check if the manual readings concur with the readings displayed on the incubator. If the incubator has a trace readout, check the printout for fluctuations in CO2 level. Check the settings to ensure that CO2 levels are set at appropriate levels for your cell line (usually between 5 and 10%). Check line connections frequently for leaks. Avoid frequent opening and closing of incubator doors. 2. Temperature fluctuations in the incubator:Monitor the temperature of incubator with a good thermometer inside the incubator. 3. Amphotericin B or other preventive antibiotics/antimycotics are present at toxic concentrations:Use at recommended levels. 4. Humidity is incorrect:Check the water level in the water pan. Humidity is vital to appropriate gas exchange for many types of cells and media. 5. Incorrect osmotic pressure in medium:Check osmolality of complete medium. Most mammalian cells can tolerate an osmolality of 260 to 350 mOsm/kg. Additions of reagents such as HEPES and drugs may affect osmolality. 6. Contamination by microorganisms:Bacterial and fungal contaminations are usually easily visible; symptoms of mycoplasma contamination are more subtle, and careful monitoring of culture morphology and regular testing are necessary to detect this type of contamination. 7. Inappropriate medium is being used:Double-check that the medium used is appropriate for your cell type and culture application. For example, ensure that the medium being used for serum-free culture is actually designed for serum-free culture; make sure that appropriate selective drugs are used at appropriate levels; check the expiration dates for the reagents being used; and store medium at appropriate temperatures in the dark.
