BT-474 [BT474]

Name: BT-474 [BT474]
Brand: Procell
SKU: CL-0040
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0040

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CL-0040

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	BT-474 [BT474]
Synonyms	Bt-474;BT474
Cellosaurus Accession	CVCL_0179
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	breast cancer cells
Tissue	Breast; Duct; Mammary gland
Age	60Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. BT-474 cells proliferate slowly and mainly grow in sheets. 4. Subculture when confluence reaches 80%. Cells do not reach full confluence; subculture every 5–6 days. 5. A small amount of dark granules may appear during cell culture.
Complete Medium	RPMI-1640 [PM150110]+10 μg/mL Insulin [PB180432]+2 mM L-glutamine [PB180420]+20% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	BT-474 [BT474] cells were isolated from a solid invasive ductal carcinoma of the breast by E. Lasfargues and W.G. Coutinho.
Tumorigenic	Yes, in Amsterdam/IMR rats with regression in 10 days. Yes, in nude mice.
Biosafety Level	BSL-1