Caco-2

Name: Caco-2
Brand: Procell
SKU: CL-0050
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0050

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CL-0050

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	Caco-2
Synonyms	CaCo-2;CACO-2;Caco 2;CACO 2;CACO2;CaCo2;CaCO2;Caco2;Caco-2/ATCC
Cellosaurus Accession	CVCL_0025
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	colorectal cancer cells
Tissue	Colon
Age	72Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Cells exhibit slow adherence, especially shortly after recovery; they generally adhere and spread out within 2–3 days, and vacuoles may be present. 4. Ensure thorough cell dispersion during subculture; otherwise, cells will have difficulty adhering. 5. Cells grow in sheets; the higher the cell density, the more vacuoles and dark granules appear on the cell surface. 6. Subculture is generally performed when cells reach 80% confluence. Cells form sheets, indicating a high actual density. 7. Over-confluence severely impacts cell condition; cells become fragile and die after subculture. 8. The longer the culture duration, the more refractory cells are to dissociation. Terminate dissociation when most cells are visually observed to have detached. With short culture time and low density, cells can be dissociated into single cells within 3–5 minutes at 37°C. Cells cultured for more than 3 days with density above 70% are relatively refractory to dissociation, generally requiring 5–10 minutes.
Complete Medium	MEM, with NEAA [PM150410]+20% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:6
Medium Renewal	2 to 3 times per week
Dissociation Duration	5-10 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	Caco-2 cells were isolated from a primary colonic adenocarcinoma. When grown to confluence, Caco-2 cells exhibit characteristic enterocytic differentiation. They express both cellular retinoic acid-binding protein I and II and are positive for cytokeratin.
Tumorigenic	Yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with colonic primary (grade II).
Receptor Expression	This cell line expressed heat stable enterotoxin (Sta, E. coli) and epidermal growth factor (EGF).
Gene Expression	keratin, retinoic acid binding protein 1, retinol binding protein 2
Biosafety Level	BSL-1