CEM/C1

Name: CEM/C1
Brand: Procell
SKU: CL-0328
Price: 460.0 USD
Availability: InStock

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Cat. No.: CL-0328

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CL-0328

$ 460.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	CEM/C1
Synonyms	CCRF-CEM C1;CEM-C1;CEM.C1;CEMC1
Cellosaurus Accession	CVCL_3496
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	leukemia cells
Tissue	Peripheral blood
Age	4Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

CEM/C1 cells are a camptothecin (CPT)-resistant subline of the human T-cell leukemia cell line CCRF-CEM (CCRF CEM). Selected and subcloned for CPT resistance in 1991, these cells exhibit cross-resistance to the water-soluble CPT analog topotecan as well as the water-insoluble analogs 9-amino-CPT and 10,11-methylenedioxy-CPT. CEM/C1 cells are approximately 31-fold less sensitive to CPT than the parental CEM cells. They display an atypical multidrug resistance phenotype and altered topoisomerase I catalytic activity, with CPT resistance maintained for more than six months.

Biosafety Level

BSL-1