COV434

Name: COV434
Brand: Procell
SKU: CL-0527
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0527

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CL-0527

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	COV434
Synonyms	COV-434;COV 434
Cellosaurus Accession	CVCL_2010
Organism	Human
Growth Properties	Adherent with clustering
Morphology	Epithelial-like
Disease	ovarian cancer cells
Tissue	Ovary
Age	26Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. At 24 h post-recovery, cells are not yet fully spread. Most cells remain as round, translucent cell clusters and are predominantly adherent. Occasionally, a small number of cell clusters may float; medium change is not required at this time. At 48 h post-recovery, cells attached to the bottom of the culture vessel will spread out and exhibit a cobblestone morphology. However, some round cell clusters may still adhere to the underlying cell monolayer. Medium change can be performed at this point. 4. During recovery, maintain an appropriate gas atmosphere and medium volume to ensure proper pH of the culture medium. Suboptimal conditions may prevent cell adherence.
Complete Medium	DMEM [PM150210]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.