D-Hank's Balanced Salt Solution (D-HBSS), without calcium, magnesium, phenol red
Publications(22)
Manual
PB180321
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Product Introduction
Background
Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution.
Hank's Balanced Salt Solution (HBSS) is one of the commonly used phosphate buffers in cell separation or culture. The main components are NaCl、KCl、KH₂PO₄、Na₂HPO₄、NaHCO₃ and glucose. HBSS is used for the washing of cells before passage and tissue dissociation, preparation of cell dissociation reagent, transport of cell or tissue, dilution of cells, etc.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 350 mg/L NaHCO₃
Without
• Phenol red
• Ca²⁺
• Mg²⁺
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's D-Hank's Balanced Salt Solution (D-HBSS), without calcium, magnesium, phenol red complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 1 EU/mL |
| pH | 7.0-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 36 months |
| Storage Condition | This product can be stored at 2-30℃ for 36 months. |
| Shipping Condition | Room Temperature |
Documents
Publications
Journal: Frontiers in Cell and Developmental Biology (2025) IF: 4.3
DOI: 10.3389/fcell.2025.1622998
Product Cited: D-Hank's Balanced Salt Solution (D-HBSS), without calcium, magnesium, phenol red, Fetal Bovine Serum
Journal: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A (2025) IF: 3.9
DOI: 10.1002/jbm.a.37973
Product Cited: Phosphate Buffer (PBS, 1 ×), D-Hank's Balanced Salt Solution (D-HBSS), without calcium, magnesium, phenol red
Journal: LUNG (2025) IF: 3.9
DOI: 10.1007/s00408-025-00850-2
Product Cited: Ham's F-12K Medium, A549 Cells Line, D-Hank's Balanced Salt Solution (D-HBSS), without calcium, magnesium, phenol red
Journal: EXPERIMENTAL EYE RESEARCH (2025) IF: 2.7
DOI: 10.1016/j.exer.2025.110530
Product Cited: D-Hank's Balanced Salt Solution (D-HBSS), without calcium, magnesium, phenol red
Journal: EXPERIMENTAL EYE RESEARCH (2025) IF: 2.7
DOI: 10.1016/j.exer.2025.110743
Product Cited: D-Hank's Balanced Salt Solution (D-HBSS), without calcium, magnesium, phenol red, Krebs-Ringer Solution
FAQs
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Q:1 What is the difference between DPBS and D-HBSS?
AnswerDPBS mainly contains a phosphate buffer system, sodium chloride, potassium ions, calcium ions, magnesium ions, and a small amount of glucose. Its phosphate content is relatively lower than that of PBS, and depending on the specific formulation, it may not contain calcium and magnesium ions to meet specific cell culture requirements and reduce potential interference of calcium and magnesium ions in cell experiments. D-HBSS is also a buffered salt solution designed to mimic extracellular fluid ion concentrations. It contains sodium, potassium, chloride, phosphate, bicarbonate, glucose, etc., but it typically does not contain calcium and magnesium ions. D-HBSS is sometimes specifically labeled as calcium-and-magnesium-free (D-Hanks) to suit experiments that are sensitive to calcium and magnesium ions. DPBS is widely used for cell washing, preparation of cell culture media, cell resuscitation, washing prior to cryopreservation, and as a diluent in experiments. Its flexible formulation makes it suitable for the maintenance and processing of various types of cells. D-HBSS is commonly used for the short-term maintenance of cells, temporary washing during medium changes, or as a supporting solution for short-term cell survival in certain experiments, especially those requiring strict control of calcium and magnesium ion concentrations.
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Q:2 What is the difference between D-Hank's and Hank's Balanced Salt Solution?
AnswerD-Hank's Balanced Salt Solution is a form of Hank's Balanced Salt Solution (HBSS) that does not contain calcium or magnesium ions. It is one of the commonly used phosphate-buffered solutions in cell isolation or culture. Its main components include NaCl, KCl, KH₂PO₄, Na₂HPO₄, NaHCO₃, and glucose. D-Hank's is typically used for washing cells prior to passaging or tissue dissociation, preparing cell dissociation solutions, transporting cells or tissues, and as a diluent for cell counting. Hank's Balanced Salt Solution (HBSS), on the other hand, contains calcium and magnesium ions. It is also commonly used in cell isolation or culture, with major components including NaCl, KCl, KH₂PO₄, Na₂HPO₄, NaHCO₃, CaCl₂, MgCl₂, MgSO₄, and glucose. HBSS is generally used for rinsing tissue samples, washing cells, transporting cells or tissues, preparing other reagents, and as a diluent for cell counting.
