Publications(1872)
Manual
PM150210
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Product Introduction
Background
DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times.
The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture.
DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production.
DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 4500 mg/L D-Glucose
• 4 mM L-Glutamine
• 3700 mg/L NaHCO₃
• 15 mg/L Phenol red
• 1 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's DMEM (High glucose) also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL 1000mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 1 EU/mL |
| pH | 7.0-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Documents
Publications
Journal: MEDIATORS OF INFLAMMATION (2021) IF: 4.711
DOI: 10.1155/2021/8086253
Product Cited: DMEM (High glucose) Medium
Journal: Toxicology (2021) IF: 4.5
DOI: 10.1016/j.tox.2021.152805
Product Cited: DMEM(High glucose), Penicillin-Streptomycin Solution, 100×, DMEM(High glucose), without phenol red
Journal: OncoTargets and Therapy (2021) IF: 4.4
DOI: 10.2147/OTT.S279942
Product Cited: 293T [HEK-293T] Cells Line, DMEM(High glucose), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: Drug Design Development and Therapy (2021) IF: 4.319
DOI: 10.2147/DDDT.S317701
Product Cited: Fetal Bovine Serum, DMEM (High glucose) Medium, LX-2 Cells Line
Journal: Journal Of Dairy Science (2021) IF: 4.2
Product Cited: DMEM(High glucose), PUMC-HUVEC-T1 Cells Line
FAQs
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Q:1 Can basic culture medium be used if it is accidentally frozen at -20°C?
AnswerIf the cell culture medium is accidentally frozen, allow it to thaw completely at room temperature and gently mix before use. After thawing, check whether any precipitation has formed. If no precipitate is observed, the medium can be used normally. If precipitation is present, it indicates that certain components have precipitated out of solution; in this case, to avoid affecting cell growth, the medium is not recommended for further use.
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Q:2 What is the difference between DMEM high- glucose and low-glucose formulations, and how should one choose?
AnswerThe primary difference between high-glucose and low-glucose DMEM formulations is the glucose concentration. The choice should be based on the metabolic requirements of the cell line and the experimental objectives. High-glucose DMEM contains 4.5 g/L (25 mM) glucose, providing an abundant carbon source. It is suitable for metabolically active, rapidly proliferating cell lines, including many tumor cells, and supports high-density and long-term culture without rapid nutrient depletion. Low-glucose DMEM contains 1.0 g/L (5.6 mM) glucose, which is closer to physiological blood glucose levels. It is more appropriate for cells with lower metabolic rates, certain primary cell cultures, or experimental conditions that require physiological relevance, such as modeling normoglycemic conditions or studying glucose restriction. It is also suitable for cells sensitive to high glucose or for low-glucose stress studies.
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Q:3 Can I culture all my cells in DMEM medium?
AnswerIt is not recommended to culture all cell types in DMEM, as different cell lines have specific nutritional requirements and growth characteristics. Using an inappropriate medium may result in poor cell health, reduced proliferation, or even cell death. Although DMEM is widely used, its high-glucose formulation (4.5 g/L) and elevated amino acid content are not suitable for all cell types. For example, suspension lymphocyte cell lines such as Raji and Jurkat are better maintained in RPMI 1640 medium, whereas insect cell lines such as Sf9 require entirely different media, such as Grace’s or TNM-FH. In addition, primary cells and stem cells often require specific growth factors, hormones, or specialized commercial media to maintain their undifferentiated state and normal physiological functions. Therefore, when selecting a culture medium, it is essential to consult the official guidelines provided by authoritative cell repositories such as ATCC and DSMZ, and to strictly follow the recommended basal medium, serum concentration, and supplementation conditions optimized for the specific cell line.
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Q:4 Can Neurobasal Medium alone or with B-27 Supplement be frozen to extend the shelf life of the product?
AnswerFreezing the culture medium is not recommended because a precipitate may form upon thawing; the inorganic salts and amino acids in the formulation may come out of solution when the temperature fluctuates, and once formed, these precipitates do not easily return to solution.
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Q:5 If the basic culture medium is frozen at -20℃, will it affect its use?
AnswerIf the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
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Q:6 My cells are growing very slowly. What could be the cause of this?
AnswerPlease see some common reasons below, and solutions to the issue: - Growth medium is not correct: Use pre-warmed growth medium as recommended by the supplier. - Serum in the growth medium is of poor quality: Use serum from a different lot. and choose good quality serum to ensure nutrition. - Cells have been passaged too many times: Use healthy, low-passage number cells. - Cells were allowed to grow beyond confluency: Passage mammalian cells when they are in the log-phase before they reach confluency. - Culture is contaminated with mycoplasma: Discard cells, media, and reagents. Obtain new stocks of cells, and use them with fresh media and reagents.
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Q:7 What factors can contribute to rapid cell death/culture failure?
AnswerThere are a number of events that can contribute to this: 1. Incorrect CO2 levels:Monitor the level of CO2 manually with a Fyrite kit, available from Bacharach. Check if the manual readings concur with the readings displayed on the incubator. If the incubator has a trace readout, check the printout for fluctuations in CO2 level. Check the settings to ensure that CO2 levels are set at appropriate levels for your cell line (usually between 5 and 10%). Check line connections frequently for leaks. Avoid frequent opening and closing of incubator doors. 2. Temperature fluctuations in the incubator:Monitor the temperature of incubator with a good thermometer inside the incubator. 3. Amphotericin B or other preventive antibiotics/antimycotics are present at toxic concentrations:Use at recommended levels. 4. Humidity is incorrect:Check the water level in the water pan. Humidity is vital to appropriate gas exchange for many types of cells and media. 5. Incorrect osmotic pressure in medium:Check osmolality of complete medium. Most mammalian cells can tolerate an osmolality of 260 to 350 mOsm/kg. Additions of reagents such as HEPES and drugs may affect osmolality. 6. Contamination by microorganisms:Bacterial and fungal contaminations are usually easily visible; symptoms of mycoplasma contamination are more subtle, and careful monitoring of culture morphology and regular testing are necessary to detect this type of contamination. 7. Inappropriate medium is being used:Double-check that the medium used is appropriate for your cell type and culture application. For example, ensure that the medium being used for serum-free culture is actually designed for serum-free culture; make sure that appropriate selective drugs are used at appropriate levels; check the expiration dates for the reagents being used; and store medium at appropriate temperatures in the dark.
