PM150312
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 2.5 mM L-Glutamine
• 2438 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's DMEM/F12 also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL 1000mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Documents
Publications
Journal: ACS Omega (2023) IF: 4.1
Product Cited: DMEM(High glucose), DMEM/F12, Donor Equine Serum
Journal: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY (2023) IF: 4
DOI: 10.1016/j.biocel.2023.106389
Product Cited: DMEM/F12, Donor Equine Serum, MEM Non-Essential Amino Acid Solution (NEAA), 100×, Penicillin-Streptomycin Solution, 100×, MEM(with NEAA), Fetal Bovine Serum, RPMI-1640, 200 mM L-Glutamine Solution, 100×, DMEM(High glucose)
Journal: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2023) IF: 3.9
DOI: 10.1016/j.abb.2023.109764
Product Cited: DMEM/F12
Journal: EXPERIMENTAL GERONTOLOGY (2023) IF: 3.9
DOI: 10.1016/j.exger.2023.112140
Product Cited: DMEM/F12
Journal: CELL BIOLOGY INTERNATIONAL (2023) IF: 3.9
DOI: 10.1002/cbin.12020
Product Cited: C-33 A Cells Line, Ca Ski Cells Line, SiHa Cells Line, Hela Cells Line, MEM(with NEAA), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, RPMI-1640, DMEM(High glucose), McCoy's 5A, DMEM/F12
