PM150210
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Product Introduction
Background
DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times.
The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture.
DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production.
DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 4500 mg/L D-Glucose
• 4 mM L-Glutamine
• 3700 mg/L NaHCO₃
• 15 mg/L Phenol red
• 1 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's DMEM (High glucose) also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL 1000mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.0-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Documents
Publications
Journal: BRITISH JOURNAL OF CANCER (2025) IF: 6.4
DOI: 10.1038/s41416-025-03017-1
Product Cited: DMEM (High glucose) Medium, LX-2 Cells Line, Fetal Bovine Serum, Human Gastric Mucosal Epithelial Cells, Penicillin-Streptomycin Solution, 100 ×
Journal: PHYTOTHERAPY RESEARCH (2025) IF: 6.3
DOI: 10.1002/ptr.8526
Product Cited: Fetal Bovine Serum, DMEM (High glucose) Medium
Journal: JCI Insight (2025) IF: 6.3
DOI: 10.1172/jci.insight.186871
Product Cited: DMEM (High glucose) Medium, MEM, with NEAA Medium, McCoy's 5A Medium, Ham's F-12K Medium
Journal: PHYTOTHERAPY RESEARCH (2025) IF: 6.3
DOI: 10.1002/ptr.70021
Product Cited: DMEM (High glucose) Medium, Penicillin-Streptomycin Solution, 100 ×
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2025) IF: 6.2
DOI: 10.1016/j.ecoenv.2025.118355
Product Cited: DMEM (High glucose) Medium, Penicillin-Streptomycin Solution, 100 ×, RAW 264.7 Cell Complete Medium
