EA.hy926

Name: EA.hy926
Brand: Procell
SKU: CL-0272
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0272

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CL-0272

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	EA.hy926
Synonyms	EA. hy 926;EA hy 926;EAhy 926;EAHY-926;EA.Hy926;EA.hy926;EAhy926;EaHy926;Eahy926
Cellosaurus Accession	CVCL_3901
Organism	Human
Growth Properties	Adherent
Morphology	Endothelial-like
Tissue	Umbilical vein
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line exhibits significant density dependence. It is sensitive to pH; culture dishes or vented flasks are recommended. Suboptimal conditions may result in poor adherence and increased floating cells.
Complete Medium	DMEM/F12 [PM150312]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	1-2 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	EA.hy926 cells were established by fusing primary human umbilical vein endothelial cells with a thioguanine-resistant A549 clone using PEG (polyethylene glycol). The hybrid cells were selected in HAT medium and screened for Factor VIII–related antigen. EA.hy926 cells have undergone more than 100 population doublings (PDLs). Electron micrographs show the presence of Weibel-Palade bodies and tissue-specific organelles in the cytoplasm, as well as differentiated endothelial cell features related to angiogenesis, hemostasis and thrombosis, blood pressure regulation, and inflammatory responses.
Antigen Expression	Factor VIII-related antigen; Homo sapiens, expressed
Gene Expression	Factor VIII-related antigen; Homo sapiens.
Biosafety Level	BSL-1