Ham's F-12

Cat. No.: PM150810 Publications(55) Manual
Ham's F-12 - 1
  • Ham's F-12Ham's F-12 - 1

PM150810

$ 10.00

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500mL

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Product Introduction

Background

Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum free culture. At low serum levels, it is especially suitable for single cell culture and clonal culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, the DMEM/F12 medium obtained  by mixing Ham's F-12 in equal volume with DMEM is more nutritious and widely used.

Ingredient Statement
With

• 1802 mg/L D-Glucose

• 1 mM L-Glutamine

• 1176 mg/L NaHCO₃

• 1.2 mg/L Phenol red

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Ham's F-12 complies with ISO 13485, ISO 9001.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.0-7.6
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

FTIR microspectroscopic study of gastric cancer AGS cells apoptosis induced by As2O3

Journal: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2024) IF: 4.4

DOI: 10.1016/j.saa.2024.123998

Product Cited: Ham's F-12

Inflammation-Related Gene ADH1A Regulates the Polarization of Macrophage M1 and Influences the Malignant Progression of Gastric Cancer

Journal: Journal of Inflammation Research (2024) IF: 4.2

DOI: 10.2147/JIR.S452670

Product Cited: AGS Cells Line, Fetal Bovine Serum, RPMI-1640 Medium, THP-1 Cells Line, HGC-27 Cells Line, Ham's F-12 Medium

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