PM150810
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Product Introduction
Background
Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum free culture. At low serum levels, it is especially suitable for single cell culture and clonal culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, the DMEM/F12 medium obtained by mixing Ham's F-12 in equal volume with DMEM is more nutritious and widely used.
Ingredient Statement
With
• 1802 mg/L D-Glucose
• 1 mM L-Glutamine
• 1176 mg/L NaHCO₃
• 1.2 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Ham's F-12 complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.0-7.6 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Publications
Journal: EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2023) IF: 4.600
DOI: 10.1016/j.ejps.2023.106466
Product Cited: Hela Cells Line, A549 [A-549] Cells Line, MCF7 [MCF-7] Cells Line, SMMC-7721 Cells Line, DMEM(High glucose), Penicillin-Streptomycin Solution, 100×, Ham's F-12
Journal: ACS Omega (2023) IF: 4.1
Product Cited: Eca-109 Cells Line, KYSE-150 Cells Line, RPMI-1640, Penicillin-Streptomycin Solution, 100×, Ham's F-12
Journal: Journal of Pathology Clinical Research (2023) IF: 4.1
DOI: 10.1002/cjp2.333
Product Cited: GES-1 Cells Line, AGS Cells Line, BGC-823 Cells Line, HGC-27 Cells Line, MKN-28 Cells Line, Ham's F-12, RPMI-1640
Journal: REDOX REPORT (2023) IF: 3.8
DOI: 10.1080/13510002.2023.2251234
Product Cited: Ham's F-12
Journal: ACTA BIOCHIMICA ET BIOPHYSICA SINICA (2023) IF: 3.7
DOI: 10.3724/abbs.2023154
Product Cited: RPMI-1640 Medium, KYSE-150 Cells Line, TE-1 Cells Line, Ham's F-12 Medium, Penicillin-Streptomycin Solution, 100 ×
