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Product Introduction
| Cell Name | HCC1143 |
| Synonyms | HCC-1143;Hamon Cancer Center 1144 |
| Cellosaurus Accession | CVCL_1245 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | breast cancer cells |
| Tissue | Breast |
| Age | 52Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:5 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 4-5 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | HCC1143 is a mammary epithelial cell line isolated from a 52-year-old Caucasian female patient with invasive ductal carcinoma of the breast, TNM stage IIA, grade 3. The cell line was initiated on August 30, 1994, and required 29 months to establish. |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: DRUG RESISTANCE UPDATES (2025) IF: 21.7
DOI: 10.1016/j.drup.2025.101349
Product Cited: MCF7 Cells Line, T-47D Cells Line, HCC1143 Cells Line, HCC1954 Cells Line, Hank's Balanced Salt Solution (HBSS), with calcium, magnesium, without phenol red, MDA-MB-231 Cells Line, MDA-MB-361 Cells Line, Penicillin-Streptomycin Solution, 100 ×, MCF 10A Cells Line, SK-BR-3 Cells Line, MDA-MB-468 Cells Line, MDA-MB-436 Cells Line, HCC38 Cells Line, HCC1937 Cells Line
Journal: Stem Cell Research & Therapy (2025) IF: 7.3
DOI: 10.1186/s13287-025-04648-0
Product Cited: SK-BR-3 Cells Line, HCC1143 Cells Line, MCF 10A Cells Line, MCF7 Cells Line, MDA-MB-231 Cells Line, MDA-MB-468 Cells Line
