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CL-0768
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Product Introduction
General information
| Cell Name | HCC1428 |
| Synonyms | HCC-1428;Hamon Cancer Center 1428 |
| Cellosaurus Accession | CVCL_1252 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like with occasional vacuole formation |
| Disease | breast cancer cells |
| Tissue | Mammary gland/breast; derived from metastatic site: skin |
| Age | 49Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | HCC1428 was derived from a 49-year-old Caucasian female with breast adenocarcinoma, TNM stage IV, grade 4. The patient carried a homozygous deletion in the FHIT gene, which is also retained in the EBV-transformed lymphoblastoid cell line from the same individual (HCC1428 BL; ATCC CRL-2328). The cell line was initiated on November 4, 1995, and established after 15 months in culture. Deposited by A. F. Gazdar and A. K. Virmani, HCC1428 is an epithelial cell line suitable for cancer research. |
| Gene Expression | epithelial glycoprotein 2 [EGP2] positive; cytokeratin 19 positive; Her2-neu negative; p53 negative |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
NCBP2 Regulates PGAM5-Mediated Mitophagy Via KIF23 Alternative Splicing To Promote Cervical Cancer ProgressionRun Title: NCBP2 Promotes Cervical Cancer Via Mitophagy
Journal: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2026) IF: 3.3
DOI: 10.1007/s12010-026-05607-y
Product Cited: Hela Cells Line, SiHa Cells Line, MS751 Cells Line, HCC1428 Cells Line, C-33 A Cells Line, ME-180 Cells Line, Ca Ski Cells Line
