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Product Introduction
| Cell Name | HCC38 |
| Synonyms | Hcc38;HCC-38;HCC 38;HCC0038 |
| Cellosaurus Accession | CVCL_1267 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | breast cancer cells |
| Tissue | Breast; Duct; Mammary gland |
| Age | 50Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | HCC38 cells were established in 1992 from breast ductal carcinoma tissue of a 50-year-old Caucasian female. The patient had a prior history of leiomyosarcoma, and her mother died of breast cancer. HCC38 cells are HER2-negative and p53-positive. The epithelial cell-specific markers EGP2 (epithelial glycoprotein 2) and CK19 are positive, while estrogen receptor (ER) and progesterone receptor (PR) are negative. |
| Receptor Expression | estrogen receptor, not expressed; progesterone receptor, not expressed |
| Gene Expression | Epithelial glycoprotein 2 [EGP2]; cytokeratin 19 |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: DRUG RESISTANCE UPDATES (2025) IF: 21.7
DOI: 10.1016/j.drup.2025.101349
Product Cited: MCF7 Cells Line, T-47D Cells Line, HCC1143 Cells Line, HCC1954 Cells Line, Hank's Balanced Salt Solution (HBSS), with calcium, magnesium, without phenol red, MDA-MB-231 Cells Line, MDA-MB-361 Cells Line, Penicillin-Streptomycin Solution, 100 ×, MCF 10A Cells Line, SK-BR-3 Cells Line, MDA-MB-468 Cells Line, MDA-MB-436 Cells Line, HCC38 Cells Line, HCC1937 Cells Line
Journal: Biomolecules (2025) IF: 4.8
DOI: 10.3390/biom15060835
Product Cited: HCC38 Cells Line, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, HCC1937 Cells Line, Fetal Bovine Serum, DMEM (High glucose) Medium
Journal: CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS (2021) IF: 3.632
Product Cited: BT-549 Cells Line, MDA-MB-231 Cells Line, HCC38 Cells Line, SUM159PT Cells Line, MDA-MB-453 Cells Line, MCF 10A Cells Line
