HK-2

Name: HK-2
Brand: Procell
SKU: CL-0109
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0109

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CL-0109

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	HK-2
Synonyms	Hk-2;HK2;Human Kidney-2
Cellosaurus Accession	CVCL_0302
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Kidney; cortex; proximal tubule
Age	Adult
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	HK-2 cells are derived from normal human renal proximal tubule cells immortalized by transduction with the HPV‑16 E6/E7 genes. The recombinant retroviral vector pLXSN 16 E6/E7 was transfected into the ecotropic packaging cell line Psi‑2; virus produced by Psi‑2 cells was then used to infect the amphotropic packaging cell line PA317. Finally, viral particles generated by PA317 cells were used to transduce normal renal cortical proximal tubule cells. Although pLXSN 16 E6/E7 contains a neomycin resistance gene, G418 selection was not used to isolate transduced clones. Southern blot and FISH analyses demonstrated that HK-2 cells are derived from a single clone, and PCR analysis confirmed the presence of the E6/E7 genes in the HK-2 genome.
Receptor Expression	epidermal growth factor (EGF), expressed
Gene Expression	alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin
Biosafety Level	BSL-2