IM-9
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Cat. No.: CL-0367
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CL-0367
$ 360.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | IM-9 |
| Synonyms | IM 9;IM9;GM04680 |
| Cellosaurus Accession | CVCL_1305 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Tissue | Bone marrow |
| Age | Age unspecified |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line displays irregular morphology with minor clustering. Proliferation is relatively rapid; timely subculture is recommended. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | Half-medium change every 2 to 3 days |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The IM-9 cell line was established in 1967 from the bone marrow of an adult female patient with multiple myeloma. Although initially classified as a myeloma cell line, subsequent studies confirmed it to be an Epstein-Barr virus (EBV)-transformed B lymphoblastoid cell line. |
| Receptor Expression | growth hormone receptor; insulin receptor; calcitonin receptor |
| Antigen Expression | CD11a+; CD19+; CD20+; CD38 -; CD49e+ |
| Gene Expression | immunoglobulin |
| Biosafety Level | BSL-2 |
MSDS
