Jurkat clone A3[A3]

Name: Jurkat clone A3[A3]
Brand: Procell
SKU: CL-0315
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0315

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CL-0315

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	Jurkat clone A3[A3]
Synonyms	Jurkat clone A3
Cellosaurus Accession	CVCL_1061
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	leukemia cells
Tissue	Peripheral blood
Age	14Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-2×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

The Jurkat clone A3[A3] subclone was derived from the Jurkat cell line via treatment with Fas antibody, followed by limiting dilution to generate a single-cell clone. The A3 cell line exhibits low spontaneous resistance to Fas-mediated apoptosis and is highly sensitive to this pathway. Wild-type A3 cells with neomycin resistance were selected and treated three times with the frameshift mutagen ICR-191 to isolate recessive mutants resistant to Fas antibody-induced cell death.

Biosafety Level

BSL-1