LIM1215
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Cat. No.: CL-1122
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CL-1122
$ 460.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | LIM1215 |
| Synonyms | Lim1215;LIM 1215;LIM-1215 |
| Cellosaurus Accession | CVCL_2574 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Tissue | Colon |
| Age | 34Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. LIM1215 exhibits weak adherence and grows in an island-like morphology; these island-like structures may pile up on each other. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+0.6 μg/mL Insulin [PB180432]+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 5-6 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | LIM1215 is a human colorectal cancer cell line derived from a biopsy of a metastatic lesion in the greater omentum, with the primary tumor arising in the ascending colon. It was isolated from a 34-year-old male patient with a family history of colorectal cancer and a clinical diagnosis of hereditary nonpolyposis colorectal cancer (HNPCC). These cells harbor mutant β-catenin, secrete TGF-α, and respond to both TGF-α and EGF. They express the A33 antigen and exhibit microsatellite instability (MSI). |
| Biosafety Level | BSL-1 |
MSDS
