McCoy's 5A

Cat. No.: PM150710 Publications(254) Manual
McCoy's 5A - 1
  • McCoy's 5AMcCoy's 5A - 1

PM150710

$ 10.00

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500mL

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Product Introduction

Background

McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement
With

• 3000 mg/L D-Glucose

• 1.5 mM L-Glutamine

• 2200 mg/L NaHCO₃

• 10 mg/L Phenol red

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's McCoy's 5A also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Publications

Retraction of “Label-Free Isolation of Low-Adhesion Cells with Stem Properties for Cancer Stem Cell-Specific Drug Evaluation”

Journal: ANALYTICAL CHEMISTRY (2022) IF: 8

DOI: 10.1021/acs.analchem.2c00444

Product Cited: MCF7 [MCF-7] Cells Line, MDA-MB-231 Cells Line, SUM159PT Cells Line, B16 Cells Line, MDA-MB-435 Cells Line, SK-BR-3 [SKBR3] Cells Line, McCoy's 5A

PHGDH Inhibitor CBR-5884 Inhibits Epithelial Ovarian Cancer Progression via ROS/Wnt/β-Catenin Pathway and Plays a Synergistic Role with PARP Inhibitor Olaparib

Journal: Oxidative Medicine and Cellular Longevity (2022) IF: 7.3

DOI: 10.1155/2022/9029544

Product Cited: A2780 Cells Line, NIH:OVCAR-3 [OVCAR3] Cells Line, ES-2 Cells Line, RPMI-1640, Fetal Bovine Serum, McCoy's 5A, Enhanced Cell Counting Kit 8 (WST-8/CCK8), PI Reagent (50μg/mL)

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