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McCoy's 5A

Cat. No.: PM150710 Publications(275) Manual
McCoy's 5A - 1
  • McCoy's 5AMcCoy's 5A - 1

PM150710

$ 10.00

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500mL

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Product Introduction

Background

McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement
With

• 3000 mg/L D-Glucose

• 1.5 mM L-Glutamine

• 2200 mg/L NaHCO₃

• 10 mg/L Phenol red

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's McCoy's 5A also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Publications

ADAM10 promotes cell growth, migration, and invasion in osteosarcoma via regulating E-cadherin/β-catenin signaling pathway and is regulated by miR-122-5p

Journal: Cancer Cell International (2020) IF: 5.8

DOI: 10.1186/s12935-020-01174-2

Product Cited: Saos-2 Cells Line, SW 1353 Cells Line, HOS Cells Line, McCoy's 5A

miR-370-3p Alleviates Ulcerative Colitis-Related Colorectal Cancer in Mice Through Inhibiting the Inflammatory Response and Epithelial-Mesenchymal Transition

Journal: Drug Design Development and Therapy (2020) IF: 4.319

DOI: 10.2147/DDDT.S238124

Product Cited: McCoy's 5A Medium, SW620(DMEM Cells Line, HCT 116 Cells Line, SW620 Cells Line, HT-29 Cells Line, McCoy's 5A

Down-regulation of lncRNA BLACAT1 inhibits ovarian cancer progression by suppressing the Wnt/β-catenin signaling pathway via regulating miR-519d-3p

Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY (2020) IF: 4.3

DOI: 10.1007/s11010-020-03704-y

Product Cited: ES-2 Cells Line, McCoy's 5A, NIH:OVCAR-3 [OVCAR3] Cells Line

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