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Manual
PM150710
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Product Introduction
Background
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3000 mg/L D-Glucose
• 1.5 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's McCoy's 5A also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 1 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Documents
Publications
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025) IF: 7.7
DOI: 10.1016/j.ijbiomac.2025.142791
Product Cited: McCoy's 5A Medium, DMEM (High glucose) Medium, Phosphate Buffer (PBS, 1 ×), Fetal Bovine Serum
Journal: Journal of Translational Medicine (2025) IF: 7.5
DOI: 10.1186/s12967-025-07286-5
Product Cited: McCoy's 5A Medium
Journal: ONCOGENE (2025) IF: 7.3
DOI: 10.1038/s41388-025-03488-9
Product Cited: Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, McCoy's 5A Medium, DMEM (High glucose) Medium, CT26.WT Cells Line, SW480 Cells Line, HT-29 Cells Line
Journal: ONCOGENE (2025) IF: 6.9
DOI: 10.1038/s41388-025-03289-0
Product Cited: RPMI-1640 Medium, DMEM (High glucose) Medium, McCoy's 5A Medium, MEM, with NEAA Medium
Journal: Cell Reports (2025) IF: 6.9
DOI: 10.1016/j.celrep.2025.116581
Product Cited: U-2 OS Cells Line, McCoy's 5A Medium
FAQs
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Q:1 Can Neurobasal Medium alone or with B-27 Supplement be frozen to extend the shelf life of the product?
AnswerFreezing the culture medium is not recommended because a precipitate may form upon thawing; the inorganic salts and amino acids in the formulation may come out of solution when the temperature fluctuates, and once formed, these precipitates do not easily return to solution.
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Q:2 If the basic culture medium is frozen at -20℃, will it affect its use?
AnswerIf the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
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Q:3 My cells are growing very slowly. What could be the cause of this?
AnswerPlease see some common reasons below, and solutions to the issue: - Growth medium is not correct: Use pre-warmed growth medium as recommended by the supplier. - Serum in the growth medium is of poor quality: Use serum from a different lot. and choose good quality serum to ensure nutrition. - Cells have been passaged too many times: Use healthy, low-passage number cells. - Cells were allowed to grow beyond confluency: Passage mammalian cells when they are in the log-phase before they reach confluency. - Culture is contaminated with mycoplasma: Discard cells, media, and reagents. Obtain new stocks of cells, and use them with fresh media and reagents.
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Q:4 What factors can contribute to rapid cell death/culture failure?
AnswerThere are a number of events that can contribute to this: 1. Incorrect CO2 levels:Monitor the level of CO2 manually with a Fyrite kit, available from Bacharach. Check if the manual readings concur with the readings displayed on the incubator. If the incubator has a trace readout, check the printout for fluctuations in CO2 level. Check the settings to ensure that CO2 levels are set at appropriate levels for your cell line (usually between 5 and 10%). Check line connections frequently for leaks. Avoid frequent opening and closing of incubator doors. 2. Temperature fluctuations in the incubator:Monitor the temperature of incubator with a good thermometer inside the incubator. 3. Amphotericin B or other preventive antibiotics/antimycotics are present at toxic concentrations:Use at recommended levels. 4. Humidity is incorrect:Check the water level in the water pan. Humidity is vital to appropriate gas exchange for many types of cells and media. 5. Incorrect osmotic pressure in medium:Check osmolality of complete medium. Most mammalian cells can tolerate an osmolality of 260 to 350 mOsm/kg. Additions of reagents such as HEPES and drugs may affect osmolality. 6. Contamination by microorganisms:Bacterial and fungal contaminations are usually easily visible; symptoms of mycoplasma contamination are more subtle, and careful monitoring of culture morphology and regular testing are necessary to detect this type of contamination. 7. Inappropriate medium is being used:Double-check that the medium used is appropriate for your cell type and culture application. For example, ensure that the medium being used for serum-free culture is actually designed for serum-free culture; make sure that appropriate selective drugs are used at appropriate levels; check the expiration dates for the reagents being used; and store medium at appropriate temperatures in the dark.
