MCDB 131 (Glucose free), without L-glutamine
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ManualPM151222
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Product Introduction
Background
MCDB is a low-protein and serum-free culture medium designed for specific cells. MCDB 131 is a kind of MCDB medium designed and developed by Knedler and Ham. It was originally used for culturing human microvascular endothelial cells (HMVEC). In addition to the use of MCDB 131 medium for endothelial cells, it also be used for other cell types, such as liver cells, smooth muscle cells, cardiomyocytes.
MCDB 131 culture medium is not used in traditional medium because it contains trace elements, putrescine, adenine, thymine, and higher concentrations of amino acids and vitamins. It is often used with EGF, hydrocortisone, glutamine and low-concentration serum together, and the added concentration of serum must be determined by cell type.
Ingredient Statement
With
• 1176 mg/L NaHCO₃
• 110 mg/L Sodium Pyruvate
• 12.4 mg/L Phenol red
Without
• D-Glucose
• L-Glutamine
• HEPES
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's MCDB 131 (Glucose free), without L-glutamine complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: MOLECULAR IMMUNOLOGY (2025) IF: 3
DOI: 10.1016/j.molimm.2025.05.023
Product Cited: MCDB 131, without L-glutamine Medium
Journal: iScience (2024) IF: 4.6
DOI: 10.1016/j.isci.2024.109608
Product Cited: MCDB 131, without L-glutamine Medium
FAQs
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Q:1 If the basic culture medium is frozen at -20℃, will it affect its use?
AnswerIf the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
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Q:2 How long can I keep my culture media after supplementing with serum?
AnswerGenerally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
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Q:3 My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
AnswerWe routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
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Q:4 How light sensitive is media? Should I also be protecting it from LED light?
AnswerWhile we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
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Q:5 I see a precipitate in my medium, and a change in the pH. What should I do?
AnswerThis is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
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Q:6 Where can I find the osmolality for Medium?
AnswerThe osmolality is listed in the COA for the particular lot number of the medium.
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Q:7 Do you provide customized culture media services?
AnswerYes. We can customize culture media with reduced components or changes in the concentration of existing components.
