MEG-01

Name: MEG-01
Brand: Procell
SKU: CL-0498
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0498

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CL-0498

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	MEG-01
Synonyms	Meg-01;MEG01;Meg01
Cellosaurus Accession	CVCL_0425
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Lymphoblast-like
Disease	leukemia cells
Tissue	Bone marrow
Age	55Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:3
Medium Renewal	2 to 3 times per week
Dissociation Duration	1-2 min
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Weakly adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. For firmly adherent cells, rinse with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 4. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

MEG-01 cells were derived from the bone marrow of a patient with chronic myeloid leukemia (CML) in megakaryoblastic crisis. The cells are positive for cytoplasmic factor VIII, surface glycoprotein IIb/IIIa, periodic acid–Schiff (PAS) activity, α-naphthyl acetate esterase, and acid phosphatase, but negative for myeloperoxidase, α-naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase, and alkaline phosphatase. They stain positively with the monoclonal antibodies BA-1 (anti-B cell/granulocyte), HPL-3 (anti-glycoprotein IIb/IIIa), and 20.3 (anti-monocyte/platelet), and are negative for other lymphoid and myeloid lineage markers.