skm-1

Name: skm-1
Brand: Procell
SKU: CL-0812
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0812

Publications(3)

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	skm-1
Synonyms	SKM1
Cellosaurus Accession	CVCL_0098
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like; round; single cells
Disease	leukemia cells
Tissue	Peripheral blood
Age	76Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	skm-1 was established in 1989 from the peripheral blood of a 76-year-old patient with acute monoblastic leukemia (AML-M5) secondary to myelodysplastic syndrome (MDS).
Biosafety Level	BSL-1