Publications(65)
Manual
PB180424
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Product Introduction
Background
Amino Acids are the basic unit of protein composition and also an important component of life. According to the cell's own ability to synthesize, amino acids can be divided into three categories: essential amino acids, semi-essential amino acids, and non-essential amino acids.
MEM non-essential amino acid solution (NEAA) contain L-alanine, L-glutamic acid, L-asparaginate, L-proline, L-aspartic acid, L-serine and glycine, can effectively improve the ratio of cells culture medium, reduce the side effect of non-essential amino acid production in cell culture, and promote cell proliferation and metabolism, which is one of the commonly used additives in cell culture.
Ingredient Statement
With
• Ultrapure water Dissolvent
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
4. This product is 100 × concentrated solution. Please dilute it to to the desired concentration before use.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's MEM Non-Essential Amino Acid Solution (NEAA), 100 × complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 100× |
| Volume | 100mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 0.6-1.7 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 12 months |
| Storage Condition | This product can be stored at 2-8℃ for 12 months. |
| Shipping Condition | Ice bag |
Publications
Journal: CELLULAR SIGNALLING (2025) IF: 3.7
DOI: 10.1016/j.cellsig.2025.112049
Product Cited: MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, SK-BR-3 Cells Line, MCF7 Cells Line, Donor Equine Serum, MDA-MB-468 Cells Line, BT-474 Cells Line, MDA-MB-231 Cells Line, MCF 10A Cells Line
Journal: FOOD AND CHEMICAL TOXICOLOGY (2025) IF: 3.5
DOI: 10.1016/j.fct.2025.115686
Product Cited: Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, Fetal Bovine Serum
Journal: MOLECULAR CARCINOGENESIS (2025) IF: 3
DOI: 10.1002/mc.23912
Product Cited: MEM, with NEAA Medium, MCF7 [MCF-7] Cells Line, ZR-75-1 Cells Line, Penicillin-Streptomycin Solution, 100 ×, Donor Equine Serum, DMEM/F12 Medium, MCF 10A Cells Line, MDA-MB-453 Cells Line, 200 mM L-Glutamine Solution, 100 ×, 10 mg/mL Recombinant Human Insulin Solution, BT-474 [BT474] Cells Line, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×
Journal: Discover Oncology (2025) IF: 2.9
DOI: 10.1007/s12672-025-03586-w
Product Cited: FaDu Cells Line, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, SCC-25 Cells Line, Fetal Bovine Serum, MEM Medium, DMEM/F12 Medium, Penicillin-Streptomycin Solution, 100 ×
Journal: Frontiers in Genetics (2025) IF: 2.8
DOI: 10.3389/fgene.2025.1614254
Product Cited: MEM Non-Essential Amino Acid Solution (NEAA), 100 ×
FAQs
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Q:1 The MEM Non-Essential Amino Acids (NEAA) solution is based on the MEM formulation. Can it only be added to MEM medium, or is it also compatible with other culture media such as DMEM?
AnswerThe MEM NEAA solution can be added to media other than MEM. Since DMEM is a modified version of MEM and contains approximately twice the amino acid concentration—including non-essential amino acids—additional supplementation with NEAA is generally not necessary. However, under specific experimental conditions, such as when cells have a higher demand for amino acids or when culturing certain sensitive cell lines, it may be appropriate to supplement DMEM with MEM-derived NEAA.
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Q:2 What is the solvent used for MEM Non-Essential Amino Acids Solution (100X)?
AnswerThe solvent is purified water.
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Q:3 What is MEM Non-Essential Amino Acids?
AnswerMEM Non-Essential Amino Acids are used as a growth supplement for cell culture medium, to increase cell growth and viability. MEM Non-Essential Amino Acids contains the same non-essential amino acids found in the standard Minimum Essential Medium (MEM) at a strength of 100X.
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Q:4 What is the serial number and host for Recombinant Human Insulin Solution (10mg/ml)?
AnswerThe sequence number of this insulin is P01308, it is full-length, and the expression host is yeast.
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Q:5 If I use Pricella's B-27 (50x) to prepare Neurobasal + 2% B-27(50x), how do I prepare it? Is it 2% based on 50x or 2% based on 1x?
Answer2% based on 50×. For example, prepare 100 mL medium means adding 2 mL B-27(50×), the concentration is 2%.
