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Mono-Mac-6

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Cat. No.: CL-0836
Mono-Mac-6 - 1
  • Mono-Mac-6Mono-Mac-6 - 1
  • Mono-Mac-6Mono-Mac-6 - 2
  • Mono-Mac-6Mono-Mac-6 - 3
  • +2

CL-0836

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction

General information
Cell Name Mono-Mac-6
Synonyms MONO-MAC-6;Mono-mac-6;MONO-MAC 6;Mono Mac 6;Mono Mac6;MonoMac 6;MonoMac6;MONOMAC6;MM6
Cellosaurus Accession CVCL_1426
Organism Human
Growth Properties Suspension
Morphology Suspended single, round/pleomorphic cells or small cell clusters, sometimes loosely adherent
Disease leukemia cells
Tissue Peripheral blood
Age 64Y
Sex Male
Instructions 1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium RPMI-1640 [PM150110]+10% Nutrient+10 μg/mL Insulin [PB180432]+1% Supplement
Incubation Atmosphere Air, 95%; CO₂, 5%
Temperature 37℃
Subcultivation Ratio 1:3-1:5
Medium Renewal 2 to 3 times per week
Subculturing Procedure Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium General Freezing Medium [PB180436]
Storage Conditions For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.
Background Mono-Mac-6 was established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia (AML FAB M5) in 1985 following myeloid metaplasia; carries t(9;11)(p22;q23) leading to KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion gene.
Biosafety Level BSL-1

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