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Product Introduction
| Cell Name | NB4 |
| Synonyms | NB-4;NB.4 |
| Cellosaurus Accession | CVCL_0005 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | leukemia cells |
| Tissue | Bone marrow |
| Age | 23Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | The NB-4 cell line was established in 1989 from the bone marrow of a patient with acute promyelocytic leukemia (APL; AML FAB M3) in second relapse. |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: CELL (2026) IF: 42.5
DOI: 10.1016/j.cell.2025.12.050
Product Cited: HL-60 Cells Line, NCI-H226 Cells Line, B95-8 Cells Line, NB4 Cells Line, HMY-1 Cells Line, PC-9 Cells Line
Journal: Cell Reports (2026) IF: 6.9
DOI: 10.1016/j.celrep.2026.117042
Product Cited: MOLM-13 Cells Line, K-562 Cells Line, NB4 Cells Line
Journal: JOURNAL OF MEDICINAL CHEMISTRY (2026) IF: 6.8
DOI: 10.1021/acs.jmedchem.5c02670
Product Cited: STO Cells Line, U-937 Cells Line, NB4 Cells Line, THLE-2 Cells Line, 293T Cells Line, SW480 Cells Line, MV-4-11 Cells Line, A549 Cells Line, THLE-2 Cell Complete Medium, OCI-AML-3 Cells Line, THP-1 Cells Line, BxPC-3 Cells Line, HCT 116 Cell Complete Medium, Leibovitz's L-15 Complete Medium (10)
Journal: CELL BIOLOGY AND TOXICOLOGY (2026) IF: 5.9
DOI: 10.1007/s10565-026-10143-w
Product Cited: NB4 Cells Line, Kasumi-6 Cells Line, Human Bone Marrow Mesenchymal Stem Cells, HL-60 Cells Line, Human Bone Marrow Mesenchymal Stem Cell Complete Medium
Journal: Nature Communications (2025) IF: 15.7
DOI: 10.1038/s41467-025-66931-1
Product Cited: NB4 Cells Line
