NCI-H660

Name: NCI-H660
Brand: Procell
SKU: CL-0887
Price: 600.0 USD
Availability: InStock

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Cat. No.: CL-0887

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	NCI-H660
Synonyms	H660;H-660;NCIH660;NCI660
Cellosaurus Accession	CVCL_1576
Organism	Human
Growth Properties	Aggregated suspension with loose adherence
Morphology	Epithelial-like; suspended in clusters
Tissue	Prostate; derived from metastatic site: lymph node
Age	63Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line proliferates slowly. Subculture when cells reach a sufficient density.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+10 nM Supplement1+10 nM Supplement2+2 mM L-Glutamine [PB180420]+0.5% Supplement3+1% Supplement4
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	5×10⁵-2×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	90% FBS+10% DMSO
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	NCI-H660 is an epithelial neuroendocrine cell line that was isolated from the prostate tumor of a 63-year-old Caucasian male cancer patient.
Biosafety Level	BSL-1