NCM460

Name: NCM460
Brand: Procell
SKU: CL-0749
Price: 360.0 USD
Availability: InStock

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Cat. No.: CL-0749

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CL-0749

$ 360.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	NCM460
Synonyms	NCM-460
Cellosaurus Accession	CVCL_0460
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Colon; Mucosa
Age	68Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Cell morphology changes with the duration of cell adherence. 4. When cell confluence is too high, cell morphology will change.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The NCM460 cell line is derived from normal human colonic mucosal epithelial cells and serves as a key in vitro model for investigating human intestinal physiology and pathology. This cell line was isolated from normal transverse colon tissue with no malignant lesions. NCM460 cells display typical gastrointestinal epithelial features, including the expression of epithelial markers such as villin and secretory components, which confirm their epithelial origin. Notably, the cells retain a non‑tumorigenic phenotype, as they cannot form colonies in soft agar and fail to develop tumors in nude mice. NCM460 cells grow as a stable adherent monolayer under standard culture conditions. They are widely applied in studies on cell differentiation, drug transport, and epithelial barrier function. As a normal colonic epithelial cell model, NCM460 is commonly used to compare biological differences between normal and cancerous intestinal epithelial cells. It also provides a reliable platform to evaluate the impacts of dietary factors, drugs, and other exogenous stimuli on colonic epithelial homeostasis and lesions. Overall, this cell line is a powerful research tool for exploring gastrointestinal biology at cellular and molecular levels.
Tumorigenic	No, tested in nude mice and athymic mice
Biosafety Level	BSL-1