NK-92MI

Name: NK-92MI
Brand: Procell
SKU: CL-0533
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0533

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CL-0533

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	NK-92MI
Synonyms	NK-92 MI;NK-92 mi;NK92-MI;NK92MI;NK-92 transfected with MFG-Hil2
Cellosaurus Accession	CVCL_3755
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	lymphoma cells
Tissue	Peripheral blood
Age	50Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	MEMα [PM150422]+0.2 mM Inositol+0.1 mM Supplement1+0.02 mM Folic Acid+12.5% Nutrient2+12.5% Nutrient1+1% Supplement2
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	5×10⁵-1×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

The NK-92 cell line is an interleukin-2 (IL-2)-dependent natural killer (NK) cell line derived from peripheral blood mononuclear cells of a 50-year-old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. The NK-92MI cell line is an IL-2-independent NK subline generated from NK-92 cells via retroviral transduction. Parental NK-92 cells were transduced with human IL-2 cDNA carried by the retroviral MFG-hIL-2 vector; transduction was stable, presumably due to integration of the vector into the host genome. NK-92 cells are cytotoxic to a wide range of malignant cells; chromium-release assays confirm their ability to kill K562 and Daudi cells. NK-92 cells exhibit the following immunophenotypic characteristics: positive for surface markers CD2, CD7, CD11a, CD28, CD45, and CD54; negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34, and HLA-DR. The parental IL-2-dependent NK-92 cell line and a second IL-2-independent subline, NK-92CI, are available from the ATCC. Both NK-92MI and NK-92CI variants contain and express the hIL-2 cDNA and secrete bioactive IL-2; NK-92MI cells produce higher levels of IL-2 than NK-92CI cells, while parental NK-92 cells do not produce IL-2. A culture submitted to ATCC in September 1998 was found to be contaminated with mycoplasma; the progeny were cured of mycoplasma via 21-day treatment with BM Cyclin. Six weeks post-treatment, mycoplasma testing via Hoechst staining, PCR, and standard culture all yielded negative results.

Biosafety Level

BSL-2