OCI-AML-3

Name: OCI-AML-3
Brand: Procell
SKU: CL-0837
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0837

Publications(4)

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	OCI-AML-3
Synonyms	OCI/AML-3;OCI-AML3;OCI/AML3;OCI AML3;OCIAML3;Ontario Cancer Institute-Acute Myeloid Leukemia-3
Cellosaurus Accession	CVCL_1844
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	leukemia cells
Tissue	Peripheral blood
Age	57Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	IMDM [PM150510]+15% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	OCI-AML-3 was established from the peripheral blood of a 57-year-old man with acute myeloid leukemia (AML FAB M4) at diagnosis in 1987; cells carry an NPM1 gene mutation (type A) and the DNMT3A R882C mutation. Exome and RNA sequence data are available.
Biosafety Level	BSL-1