OE19

Name: OE19
Brand: Procell
SKU: CL-0754
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0754

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CL-0754

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	OE19
Synonyms	OE-19;JROECL 19;JROECL19;OEC19
Cellosaurus Accession	CVCL_1622
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	esophageal cancer cells
Tissue	Esophagus
Age	72Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	Every 2 to 3 days
Dissociation Duration	3-5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The OE19 cell line, also known as JROECL19, was originally one of five permanent human esophageal adenocarcinoma (EAC) cell lines established in 1993. It was derived from a stage III tumor of a 72-year-old male patient and deposited in ECACC by Drs J.C. Rockett and A. Morris (Department of Biological Sciences, University of Warwick) and Dr S.J. Darnton (Birmingham Heartlands Hospital). Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines, but rather the large cell lung cancer cell line H460, the colorectal adenocarcinoma cell line SW620, and the gastric fundus carcinoma cell line SKGT-2, respectively. However, they confirmed that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs. All nine of these verified EAC cell lines, together with their genotyping information, have been deposited with ECACC.
Tumorigenic	裸小鼠致癌
Biosafety Level	BSL-1