Panc 05.04
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Cat. No.: CL-0734
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CL-0734
$ 460.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | Panc 05.04 |
| Synonyms | Panc-05.04;Panc_05_04;Panc05.04;Panc 5.04;Panc5.04;PANC0504;Panc0504;Pa18C;PL-18;PL18 |
| Cellosaurus Accession | CVCL_1637 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | leukemia cells |
| Tissue | Pancreas |
| Age | 77Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10 μg/mL Insulin [PB180432]+15% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:4 |
| Medium Renewal | every 2 to 3 days |
| Dissociation Duration | 5-8 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The Panc 05.04 cell line is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head of the pancreas of a female patient with pancreatic adenocarcinoma. The cell line harbors a KRAS oncogene mutation at codon 12: a GGT→GAT mutation resulting in substitution of glycine with aspartic acid. |
| Tumorigenic | Yes; Yes, forms tumors in nude or SCID mice. |
| Receptor Expression | unknown |
| Antigen Expression | MHC class I +; MHC class II - Blood type B; Rh+ |
| Gene Expression | cytokeratin 7; cytokeratin 18; K-ras+; MHC class I+; MHC class II-; blood type B; Rh+ |
| Biosafety Level | BSL-1 |
MSDS
