PC-9-DDP

Name: PC-9-DDP
Brand: Procell
SKU: CL-1013
Price: 720.0 USD
Availability: InStock

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Cat. No.: CL-1013

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CL-1013

$ 720.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	PC-9-DDP
Organism	Human
Growth Properties	Adherent with slight suspension
Morphology	Mixed; round; spindle-shaped
Disease	lung cancer cells
Tissue	Lung
Age	45岁
Sex	男性

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Maintain cells at a drug concentration of 2–4 μg/mL for 1 week prior to experiments. Then withdraw the drug and culture for 2–3 passages before performing experiments.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1 μg/mL Supplement1+1% Supplement2
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:5
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-6 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

PC-9 is a cell line derived from human lung adenocarcinoma, with the tissue maintaining a differentiated phenotype. PC-9 was formerly designated PC-14. PC-14 was originally deposited at the RIKEN BioResource Center in 1989 as a cell line established from human lung adenocarcinoma (undifferentiated type). Subsequently, the RIKEN BioResource Center identified via short tandem repeat (STR) profiling that this cell line is identical to PC-9, a cell line derived from human lung adenocarcinoma (differentiated type). This misidentification predated the deposit of the cell line at RIKEN. The cell line was subsequently renamed PC-9 to reflect this authentication result.

Biosafety Level

BSL-2