PM150110
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 2000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2000 mg/L NaHCO₃
• 5 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's RPMI-1640 also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL 1000mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Documents
Publications
Journal: Frontiers in Immunology (2025) IF: 5.7
DOI: 10.3389/fimmu.2025.1565924
Product Cited: DMEM/F12 Medium, SK-OV-3 Cells Line, DMEM (High glucose) Medium, MEM, with NEAA Medium, T-47D Cells Line, NIH:OVCAR-3 [OVCAR3] Cells Line, MDA-MB-231 Cells Line, McCoy's 5A Medium, RPMI-1640 Medium
Journal: Biology Direct (2025) IF: 5.7
DOI: 10.1186/s13062-025-00641-y
Product Cited: DMEM (High glucose) Medium, RPMI-1640 Medium, MEM Medium
Journal: eFood (2025) IF: 5.7
DOI: 10.1002/efd2.70074
Product Cited: RPMI-1640 Medium
Journal: Frontiers in Immunology (2025) IF: 5.7
DOI: 10.3389/fimmu.2025.1592558
Product Cited: Hank's Balanced Salt Solution (HBSS), with calcium, magnesium, phenol red, RPMI-1640 Medium
Journal: Frontiers in Immunology (2025) IF: 5.7
DOI: 10.3389/fimmu.2025.1529710
Product Cited: Fetal Bovine Serum, 0.25% Trypsin Solution, DMEM (High glucose) Medium, MDA-MB-231 Cells Line, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, 4T1 Cells Line, Phosphate Buffer (PBS, 1 ×)
