RWPE-1

Name: RWPE-1
Brand: Procell
SKU: CL-0200
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0200

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CL-0200

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	RWPE-1
Synonyms	RWPE1
Cellosaurus Accession	CVCL_3791
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Prostate
Age	54Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	Complete culture medium for RWPE-1
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	every 2 to 3 days
Dissociation Duration	3-10 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	RWPE-1 cells were established by transforming epithelial cells from the peripheral zone of a normal adult human prostate tissue section with human papillomavirus type 18 (HPV-18). When cultured in three-dimensional Matrigel, RWPE-1 cells form acini and secrete PSA into the culture medium in response to androgens [PubMed:11170142]. RWPE-1 cells also form acini and produce PSA when injected subcutaneously into male nude mice in combination with Matrigel or stromal cells [PubMed:11304724]. RWPE-1 cells further transfected with the Ki-ras gene using the Kirsten murine sarcoma virus gave rise to the tumorigenic RWPE-2 and RWPE2-W99 cell lines [PubMed:9214605]. Additionally, treatment of RWPE-1 cells with N-methyl-N-nitrosourea (MNU) generated a series of tumorigenic cell lines that model different stages of prostate cancer progression, namely WPE1-NA22, WPE1-NB14, WPE1-NB11, and WPE1-NB26. According to the provider, RWPE-1 cells have tested negative for hepatitis B, hepatitis C, and human immunodeficiency virus.
Tumorigenic	No, in nude mice (with or without Matrigel).No, in soft agar.
Receptor Expression	androgen receptor, expressed (upregulated upon exposure to androgen)
Antigen Expression	kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen); (upon exposure to androgen)
Gene Expression	cytokeratin 18, cytokeratin 8; Tumor Supressor Gene(s): p53+, pRB+
Biosafety Level	BSL-2