SK-BR-3 [SKBR3]

Name: SK-BR-3 [SKBR3]
Brand: Procell
SKU: CL-0211
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0211

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CL-0211

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	SK-BR-3 [SKBR3]
Synonyms	SK-Br-3;Sk-Br-3;SK BR 03;SKBR-3;SKBr-3;SK-BR3;SKBr3;SkBr3;SKBR3
Cellosaurus Accession	CVCL_0033
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	breast cancer cells
Tissue	Mammary gland/breast; derived from metastatic site: pleural effusion
Age	43Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. SK-BR-3 cells may recover slowly from cryopreservation. Adherence is weak during recovery from cryopreservation; this is normal. This cell line exhibits loose adherence and floating cells throughout the first week of culture and after each subculture; this is not uncommon. 4. If abundant floating material is present, especially during the first week of growth, leave cells undisturbed for several days after initiating recovery. If much floating material remains, check viability with trypan blue. It is important to retain floating cells by gentle centrifugation and add them back to the same culture vessel along with adherent cells when changing medium. Do not separate or discard floating cells. Cells initially attach as small patches or clusters, and many cell aggregates remain in suspension. After several days, growth will extend outward from adherent cell clusters. Some cell debris is also typically present. Floating cells should always be retained by gentle centrifugation (125×g for 5–7 min) and returned to the culture vessel after medium exchange or subculture. 5. SK-BR-3 cells tend to pile up on each other. Do not trypsinize cells until they reach 70–80% confluence. If cells are allowed to overgrow, they will detach and float.
Complete Medium	McCoy's 5A [PM150710]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	SK-BR-3 [SKBR3] cells were established in 1970 by G. Trempe and L.J. Old from the pleural effusion of a 43-year-old Caucasian female with breast adenocarcinoma. Ultrastructural features include microfilaments, desmosomes, glycogen granules, large lysosomes, and bundles of cytoplasmic fibrils. SK-BR-3 cells overexpress the HER2/c-erbB-2 gene product.
Tumorigenic	Yes, in nude mice; forms poorly differentiated adenocarcinoma.
Receptor Expression	Blood Type A; Rh+; HLA A11, Bw22 (+/-), B40, B18
Biosafety Level	BSL-1