SK-MEL-1

Name: SK-MEL-1
Brand: Procell
SKU: CL-0440
Price: 460.0 USD
Availability: InStock

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Cat. No.: CL-0440

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	SK-MEL-1
Synonyms	SK-Mel-1;SK Mel 1;SK-Mel 1;SK-Mel1;SKMEL-1;SkMEL-1;SKMEL1;SK 1
Cellosaurus Accession	CVCL_0068
Organism	Human
Growth Properties	Suspension
Morphology	Spherical
Disease	melanoma cells
Tissue	Skin; derived from metastatic site: thoracic lymph duct
Age	29Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	SK-MEL-1 cells were established by Oettgen·F and colleagues from the thoracic duct of a 29-year-old Caucasian male with widespread, rapidly progressive malignant melanoma. SK-MEL-1 cells can produce melanin, and electron microscopy revealed that the pigment granules in SK-MEL-1 cells are associated with both autosynthesis and phagocytosis. Antibodies against SK-MEL-1 cells have been detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Tumorigenic	Yes, in nude mice; forms pigmented malignant melanomas; also forms tumors in the cheek pouch of cortisone treated hamsters.
Antigen Expression	Blood Type A; Rh+
Biosafety Level	BSL-1