SK-N-BE(2)

Name: SK-N-BE(2)
Brand: Procell
SKU: CL-0441
Price: 460.0 USD
Availability: InStock

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Cat. No.: CL-0441

Publications(17)

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	SK-N-BE(2)
Synonyms	SK-N-BE2;SKNBE(2);SKNBE-2;SKNBE2;SK-N-BE;SKNBE
Cellosaurus Accession	CVCL_0528
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Neuroblast-like
Disease	neuroblastoma cells
Tissue	Brain; derived from metastatic site: bone marrow
Age	2Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	MEM/F12 [PM151220]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	1-2 min
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Although suspended cells remain viable, their density is low and they may be aspirated and discarded directly. 2. Rinse the adherent cells with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 3. Seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The SK-N-BE(2) neuroblastoma cell line was established in November 1972 from a bone marrow biopsy taken from a child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy. SK-N-BE(2) cells exhibit moderate levels of dopamine-β-hydroxylase activity. It has been reported that the saturation density of SK-N-BE(2) cells exceeds 1 × 10⁶ cells/cm². The morphology of SK-N-BE(2) cells is variable, with some cells possessing long processes and others exhibiting an epithelioid appearance. The cells aggregate, form clumps, and float in the culture medium.
Tumorigenic	Yes, an inoculum of 1×10⁷ cells produced tumors in cortisonized hamster cheek pouches with 18% frequency.
Biosafety Level	BSL-1