SU-DHL-10

Name: SU-DHL-10
Brand: Procell
SKU: CL-0758
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0758

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	SU-DHL-10
Synonyms	Su-DHL-10;SUDHL10;Sudhl10;SUDHL-10;SuDHL 10;Stanford University-Diffuse Histiocytic Lymphoma-10;DHL10
Cellosaurus Accession	CVCL_1889
Organism	Human
Growth Properties	Suspension with multicellular aggregation
Morphology	Lymphoblast-like
Disease	lymphoma cells
Tissue	Lymph node
Age	25Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:3
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The SU-DHL-10 cell line is a B-lymphocyte cell line isolated in 1978 from the lymph node of a 25-year-old White male with large cell lymphoma.
Biosafety Level	BSL-1