SU-DHL-8

Name: SU-DHL-8
Brand: Procell
SKU: CL-0871
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0871

Publications(2)

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	SU-DHL-8
Synonyms	SUDHL8;SUDHL-8;SuDHL 8;Stanford University-Diffuse Histiocytic Lymphoma-8;DHL-8;DHL8
Cellosaurus Accession	CVCL_2207
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	lymphoma cells
Tissue	Pleural effusion
Age	59Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:5
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	SU-DHL-8 is a B lymphocyte cell line that was isolated in 1978 from the lymph node of a 59-year-old, White male with large cell lymphoma.
Antigen Expression	Fc, not expressed; IgMEAC, not expressed; IgGEA, not expressed; E, not expressed
Gene Expression	Ig+; IgM-, IgG-, IgA-, IgD-, Lambda-, Kappa-
Biosafety Level	BSL-1