VCaP

Name: VCaP
Brand: Procell
SKU: CL-0241
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0241

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CL-0241

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	VCaP
Synonyms	VCAP;Vcap;Vertebral Cancer of the Prostate
Cellosaurus Accession	CVCL_2235
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	prostate cancer cells
Tissue	Prostate
Age	59Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line proliferates slowly, with a doubling time of approximately 5–7 days. 4. Centrifugation is recommended during subculture, and replace with new coated culture vessels. 5. This cell line has a small number of vacuoles. 6. This cell line exhibits weak adherence, which affects cell growth. It is recommended to coat culture flasks with Poly-L-Lysine solution before culture. Please prepare Poly-L-Lysine solution (Cat. No.:PB180523) in advance.
Complete Medium	DMEM [PM150210]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:3
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The VCaP cell line was established in 1997 from a spinal metastasis in a patient with hormone-refractory prostate cancer. VCaP cells were initially propagated as xenografts in mice and subsequently adapted to in vitro culture. The cells are androgen-sensitive both in vivo and in vitro. It has recently been reported that VCaP cells may harbor the mouse xenotropic retrovirus Bxv-1 following xenografting in mice.
Tumorigenic	Yes, in nude and SCID mice.
Antigen Expression	cytokeratin-18; Homo sapiens, expressed p53 antigen; Homo sapiens, expressed prostate specific antigen (PSA); Homo sapiens, expressed prostatic acid phosphatase (PAP); Homo sapiens, expressed Rb protein; Homo sapiens, expressed
Gene Expression	cytokeratin-18; Homo sapiens, expressed, p53 antigen; Homo sapiens, expressed, prostate specific antigen (PSA); Homo sapiens, expressed, prostatic acid phosphatase (PAP); Homo sapiens, expressed, Rb protein; Homo sapiens, expressed
Biosafety Level	BSL-2